Composite

Part:BBa_K2424002:Design

Designed by: Eirik Bager Sundmark   Group: iGEM17_UiOslo_Norway   (2017-10-22)


nmt1+sfGFP+cyc1 in S. Pombe


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was made with the RCF10-standard in mind, to ensure it would fulfill the criteria for parts submission in iGEM. We also had to ensure that this would be able to fit with expression vectors used in S. Pombe as well.


Source

This part consists of a combination of T796A NMT1 (BBa_K2424000), S. Pombe codon optimzed superfolder GFP (BBa_K2424001) and the CYC1-terminator (BBa K2110000). For the origin of each of these sequences, please refer to the individual parts pages.

References